2025-09-18
科學聚焦：利用肉桂葉製備的奈米乳化液及其副產品改善大鼠阿茲海默症和巴金森症
Improving Alzheimer’s Disease and Parkinson’s Diseasein Rats with Nanoemulsion and Byproducts Prepared fromCinnamon LeavesBing-Huei Chen 1,*, Chen-Te Jen 2, Chia-Chuan Wang 3 and Min-Hsiung Pan 2,*1 Department of Food Science, Fu Jen Catholic University, New Taipei City 242062, Taiwan2 Graduate Institute of Food Science, National Taiwan University, Taipei City 106319, Taiwan;d11641002@ntu.edu.tw3 School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei City 242062, Taiwan;050814@mail.fju.edu.tw* Correspondence: 002622@mail.fju.edu.tw (B.-H.C.); mhpan@ntu.edu.tw (M.-H.P.)AbstractBackground/Objectives: Cinnamon leaves, an important source of the functional com-pound cinnamaldehyde (CA), have been shown to be effective in improving type II dia-betes and Parkinson’s disease (PD) in rats following the incorporation of cinnamon leafextract into a nanoemulsion. However, the effect of a cinnamon leaf extract nanoemulsion(CLEN) on improving Alzheimer’s disease (AD), the most prevalent type of dementia,remains unexplored. The objectives of this study were to determine functional com-pounds in cinnamon leaves by UPLC-MS/MS, followed by the preparation of a nanoemul-sion and its byproducts to study their effects on AD and PD in rats. Methods: Oven-dried(60°C for 2 h) cinnamon leaf powder and hydrosol, obtained by steam distillation of cin-namon leaf powder, were stored at 4°C. After determination of basic composition (crudeprotein, crude fat, carbohydrate, moisture and ash) of cinnamon leaf powder, it was ex-tracted with 80% ethanol with sonication at 60°C for 2 h and analyzed for bioactive com-pounds by UPLC-MS/MS. Then, the CLEN was prepared by mixing cinnamon leaf extractrich in CA with lecithin, soybean oil, tween 80 and ethanol in an optimal ratio, followedby evaporation to form thin-film and redissolving in deionized water. For characteriza-tion, mean particle size, polydispersity index (PDI), zeta potential, encapsulation effi-ciency, and surface morphology were determined. Animal experiments were done by di-viding 90 male rats into 10 groups (n=9), with group 2-8 being subjected to mini-osmoticpump implantation surgery in brain to infuse Amyloid-beta 40 (Aβ40) solution in groups2-8 for induction of AD, while groups 9 and 10 were pre-fed respectively with cinnamonpowder in water (0.5 g/10 mL) and in hydrosol for 4 weeks, followed by induction of ADas shown above. Different treatments for a period of 4 weeks included groups 1-9, withgroup 1 (control) and group 2 feeding with sterilized water, while groups 3, 4 and 5 werefed respectively with high (90 mg/kg), medium (60 mg/kg) and low (30 mg/kg) doses ofcinnamon leaf extracts, groups 6, 7 and 8 fed respectively with high (90 mg/kg),medium (60 mg/kg) and low (30 mg/kg) doses of nanoemulsions, groups 9 and 10 fedrespectively with 10 mL/kg of cinnamon powder in water and hydrosol (0.5 g/10 mL).Morris water maze test was conducted to determine short-term memory, long-termmemory and space probing of rats. After sacrificing of rats, brain and liver tissues werecollected for determination of Aβ40, BACE1 and 8-oxodG in hippocampi, and AchE andmalondialdehyde (MDA) in cortices, antioxidant enzymes (SOD, CAT, GSH-Px) andMDA in both cortices and livers, and dopamine in brain striata by using commercial kits. 教授表示：「此篇論文為我學術生涯最具挑戰性的一篇，從論文完成經過審查修正到接受已經過了8個月。」
https://www.mdpi.com/1999-4923/17/9/1200
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